Review



native tsp  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    Proteintech native tsp
    Fig. 1. Thermal stress induces <t>TSP-1</t> expression and tetraspanin web-like structure formation. (A) Representative bright field and epifluorescence images showing expression of an integrated <t>transgene</t> <t>tsp-1p::tsp-1::GFP</t> under temperatures and durations indicated. Scale bar, 100 μm. (B) Quantification of TSP-1::GFP fluorescence inten- sities under conditions indicated. ***P < 0.001 (n > 40 animals per condition). (C) Schematic of the tsp-1 gene structure, predicted membrane topology (by DeepTMHMM), and structure (by AlphaFold2) of TSP-1. (D) SDS-PAGE and native-PAGE Western blots showing heat-induced increase in abundance and formation of dimers and multimers (asterisk) by TSP-1::GFP. (E) Representative confocal fluorescence images showing high-resolution Z-stack views of tetraspanin web structure formed by TSP-1::GFP, with en- larged view at right bottom. Scale bar, 10 μm. a.u., arbitrary units.
    Native Tsp, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/native tsp/product/Proteintech
    Average 95 stars, based on 74 article reviews
    native tsp - by Bioz Stars, 2026-03
    95/100 stars

    Images

    1) Product Images from "Early-life stress triggers long-lasting organismal resilience and longevity via tetraspanin."

    Article Title: Early-life stress triggers long-lasting organismal resilience and longevity via tetraspanin.

    Journal: Science advances

    doi: 10.1126/sciadv.adj3880

    Fig. 1. Thermal stress induces TSP-1 expression and tetraspanin web-like structure formation. (A) Representative bright field and epifluorescence images showing expression of an integrated transgene tsp-1p::tsp-1::GFP under temperatures and durations indicated. Scale bar, 100 μm. (B) Quantification of TSP-1::GFP fluorescence inten- sities under conditions indicated. ***P < 0.001 (n > 40 animals per condition). (C) Schematic of the tsp-1 gene structure, predicted membrane topology (by DeepTMHMM), and structure (by AlphaFold2) of TSP-1. (D) SDS-PAGE and native-PAGE Western blots showing heat-induced increase in abundance and formation of dimers and multimers (asterisk) by TSP-1::GFP. (E) Representative confocal fluorescence images showing high-resolution Z-stack views of tetraspanin web structure formed by TSP-1::GFP, with en- larged view at right bottom. Scale bar, 10 μm. a.u., arbitrary units.
    Figure Legend Snippet: Fig. 1. Thermal stress induces TSP-1 expression and tetraspanin web-like structure formation. (A) Representative bright field and epifluorescence images showing expression of an integrated transgene tsp-1p::tsp-1::GFP under temperatures and durations indicated. Scale bar, 100 μm. (B) Quantification of TSP-1::GFP fluorescence inten- sities under conditions indicated. ***P < 0.001 (n > 40 animals per condition). (C) Schematic of the tsp-1 gene structure, predicted membrane topology (by DeepTMHMM), and structure (by AlphaFold2) of TSP-1. (D) SDS-PAGE and native-PAGE Western blots showing heat-induced increase in abundance and formation of dimers and multimers (asterisk) by TSP-1::GFP. (E) Representative confocal fluorescence images showing high-resolution Z-stack views of tetraspanin web structure formed by TSP-1::GFP, with en- larged view at right bottom. Scale bar, 10 μm. a.u., arbitrary units.

    Techniques Used: Expressing, Fluorescence, Membrane, SDS Page, Clear Native PAGE, Western Blot

    Fig. 2. Expression and roles of TSP-1 in membrane barrier integrity in adults and during aging. (A to D) Representative epifluorescence images showing TSP-1::GFP up-regulation from L4 (A) to young adults (B) and during aging (C) and (D). (E) Quantification of fluorescence intensities of TSP-1::GFP under conditions indicated. ***P < 0.001 (n > 30 animals per condition). (F) Schematic of the assay for acute membrane barrier functions. (G) Quantification of intensities of fluorescein accumulated by live animals under conditions indicated. ***P < 0.001 (n > 20 animals per condition). (H to J) Representative epifluorescence images showing fluorescein accumulation in animals of indicated stages, temperature and genotypes (wild-type and tsp-1 deletion mutant allele ok3594). Scale bars, 100 μm.
    Figure Legend Snippet: Fig. 2. Expression and roles of TSP-1 in membrane barrier integrity in adults and during aging. (A to D) Representative epifluorescence images showing TSP-1::GFP up-regulation from L4 (A) to young adults (B) and during aging (C) and (D). (E) Quantification of fluorescence intensities of TSP-1::GFP under conditions indicated. ***P < 0.001 (n > 30 animals per condition). (F) Schematic of the assay for acute membrane barrier functions. (G) Quantification of intensities of fluorescein accumulated by live animals under conditions indicated. ***P < 0.001 (n > 20 animals per condition). (H to J) Representative epifluorescence images showing fluorescein accumulation in animals of indicated stages, temperature and genotypes (wild-type and tsp-1 deletion mutant allele ok3594). Scale bars, 100 μm.

    Techniques Used: Expressing, Membrane, Fluorescence, Mutagenesis

    Fig. 3. ELTS-triggered TSP-1::GFP is long-lasting and requires CBP-1. (A) Representative epifluorescence images showing long-lasting up-regulation of TSP-1::GFP upon transient ELTS (T28 for 24 hours at L4). (B) Representative epifluorescence images showing up-regulation of TSP-1::GFP upon sustained thermal stress (T28 for 24, 48, or 72 hours starting at L4). (C) Quantification of fluorescence intensities of TSP-1::GFP under conditions indicated. ***P < 0.001 (n > 30 animals per condition). (D) Schematic of experimental flow using RNAi screen to identify genes required for ELTS induction of TSP-1::GFP. TFs, transcription factors. (E) Representative bright-field and epifluorescence images showing that RNAi against tsp-1 or cbp-1 blocks up-regulation of TSP-1::GFP by ELTS. (F) Quantitative RT-PCR measurements of tsp-1 expres- sion levels under conditions indicated, showing transient tsp-1 up-regulation by ELTS in a CBP-1–dependent manner. **P < 0.01 and ***P < 0.001 (three independent biological replicates). Scale bars, 100 μm. n.s., not significant.
    Figure Legend Snippet: Fig. 3. ELTS-triggered TSP-1::GFP is long-lasting and requires CBP-1. (A) Representative epifluorescence images showing long-lasting up-regulation of TSP-1::GFP upon transient ELTS (T28 for 24 hours at L4). (B) Representative epifluorescence images showing up-regulation of TSP-1::GFP upon sustained thermal stress (T28 for 24, 48, or 72 hours starting at L4). (C) Quantification of fluorescence intensities of TSP-1::GFP under conditions indicated. ***P < 0.001 (n > 30 animals per condition). (D) Schematic of experimental flow using RNAi screen to identify genes required for ELTS induction of TSP-1::GFP. TFs, transcription factors. (E) Representative bright-field and epifluorescence images showing that RNAi against tsp-1 or cbp-1 blocks up-regulation of TSP-1::GFP by ELTS. (F) Quantitative RT-PCR measurements of tsp-1 expres- sion levels under conditions indicated, showing transient tsp-1 up-regulation by ELTS in a CBP-1–dependent manner. **P < 0.01 and ***P < 0.001 (three independent biological replicates). Scale bars, 100 μm. n.s., not significant.

    Techniques Used: Fluorescence, Quantitative RT-PCR

    Fig. 4. Subcellular localization and stability of tetraspanin web formed by endogenous TSP-1::wrmScarlet. (A) Representative confocal images showing colocaliza- tion of the marker Akt-PH::GFP, which binds to intestinal apical plasma membrane inner leaflet PIP2/PIP3, with endogenous TSP-1 tagged with wrmScarlet by CRISPR. Scale bars, 10 μm. (B) Fluorescent intensity correlation analysis showing close proximity of Akt-PH::GFP and TSP-1::wrmScarlet. (C) Representative confocal images showing non-colocalization of the marker ERm::GFP, which labels intestinal ER membrane, with endogenous TSP-1 tagged with wrmScarlet by CRISPR. Scale bars, 10 μm. (D) Fluo- rescent intensity correlation analysis for ERm::GFP and TSP-1::wrmScarlet. (E) Representative confocal time-series images showing stability of tetraspanin webs formed by endogenous TSP-1::wrmScarlet, with enlarged views in (F). Scale bars, 10 μm.
    Figure Legend Snippet: Fig. 4. Subcellular localization and stability of tetraspanin web formed by endogenous TSP-1::wrmScarlet. (A) Representative confocal images showing colocaliza- tion of the marker Akt-PH::GFP, which binds to intestinal apical plasma membrane inner leaflet PIP2/PIP3, with endogenous TSP-1 tagged with wrmScarlet by CRISPR. Scale bars, 10 μm. (B) Fluorescent intensity correlation analysis showing close proximity of Akt-PH::GFP and TSP-1::wrmScarlet. (C) Representative confocal images showing non-colocalization of the marker ERm::GFP, which labels intestinal ER membrane, with endogenous TSP-1 tagged with wrmScarlet by CRISPR. Scale bars, 10 μm. (D) Fluo- rescent intensity correlation analysis for ERm::GFP and TSP-1::wrmScarlet. (E) Representative confocal time-series images showing stability of tetraspanin webs formed by endogenous TSP-1::wrmScarlet, with enlarged views in (F). Scale bars, 10 μm.

    Techniques Used: Marker, Clinical Proteomics, Membrane, CRISPR

    Fig. 5. ELTS-triggered thermal resilience and longevity requires TSP-1. (A) Life-span curves of wild-type and tsp-1 mutants at 28°C starting at L4. A total of 20% me- dian and 28% maximal survival decrease in tsp-1 mutants. ***P < 0.001 (n > 40 animals per condition). (B) Life-span curves of wild-type and tsp-1 mutants at constant 20°C or after transient 28°C for 24 hours at L4 (ELTS). A total of 36% median and 20% maximal life-span extension by ELTS in wild type. *** P < 0.001 (n > 35 animals per condition). (C) Life-span curves of wild-type and tsp-1 mutants at 28°C after transient 28°C for 24 hours at L4 (ELTS). A total of 33% median and 33% maximal survival de- crease in tsp-1 mutants. ***P < 0.001 (n > 35 animals per condition). (D) Life-span curves of wild-type animals with RNAi against cbp-1 or control at 28°C starting at L4. A total of 80% median and 50% maximal survival decrease with RNAi against cbp-1. ***P < 0.001 (n > 40 animals per condition). (E) Representative confocal fluorescence images showing ELTS-induced increase in numbers of nuclei with GFP::CBP-1 in the posterior intestine. (F) Quantification of numbers of nuclei with GFP::CBP-1 in the posterior intestine under conditions indicated. (G) Model of how ELTS induces lasting organismal thermal resilience through HSF-independent epigenetic regulation of TSP-1 and stable tetraspanin web structure formation.
    Figure Legend Snippet: Fig. 5. ELTS-triggered thermal resilience and longevity requires TSP-1. (A) Life-span curves of wild-type and tsp-1 mutants at 28°C starting at L4. A total of 20% me- dian and 28% maximal survival decrease in tsp-1 mutants. ***P < 0.001 (n > 40 animals per condition). (B) Life-span curves of wild-type and tsp-1 mutants at constant 20°C or after transient 28°C for 24 hours at L4 (ELTS). A total of 36% median and 20% maximal life-span extension by ELTS in wild type. *** P < 0.001 (n > 35 animals per condition). (C) Life-span curves of wild-type and tsp-1 mutants at 28°C after transient 28°C for 24 hours at L4 (ELTS). A total of 33% median and 33% maximal survival de- crease in tsp-1 mutants. ***P < 0.001 (n > 35 animals per condition). (D) Life-span curves of wild-type animals with RNAi against cbp-1 or control at 28°C starting at L4. A total of 80% median and 50% maximal survival decrease with RNAi against cbp-1. ***P < 0.001 (n > 40 animals per condition). (E) Representative confocal fluorescence images showing ELTS-induced increase in numbers of nuclei with GFP::CBP-1 in the posterior intestine. (F) Quantification of numbers of nuclei with GFP::CBP-1 in the posterior intestine under conditions indicated. (G) Model of how ELTS induces lasting organismal thermal resilience through HSF-independent epigenetic regulation of TSP-1 and stable tetraspanin web structure formation.

    Techniques Used: Control, Fluorescence

    Fig. 6. Gain of function of TSP-1 confers longevity extension in C. elegans and thermal resilience in human cells. (A) Schematic of the transgene that produces tsp-1 gain-of-function OE lines (OE line 1 at low level, OE line 2 at high level as measured by qRT-PCR). ***P < 0.001. (B) Life-span curves of wild type and two representative tsp-1 OE lines at 28°C starting at L4. A total of 50% median and 16% maximal survival extension by overexpression of tsp-1 in wild type, ***P < 0.001 (n > 40 animals per condition). (C) Life-span curves of wild-type and tsp-1 OE lines at constant 20°C. A total of 63% median and 24% maximal life-span extension by overexpression of tsp-1 in wild type, ***P < 0.001 (n > 40 animals per condition). (D) Serial confocal fluorescence images showing tetraspanin web-like structures formed in the human cell line HEK293 by CMV promoter-driven expression of C. elegans tsp-1::GFP (bottom) but not GFP alone (top). Scale bar, 10 μm. (E) Quantification of survival rates after heat shock in HEK293 cells showing enhanced thermal resilience conferred by ectopic gain-of-function of C. elegans TSP-1::GFP. ***P < 0.001 (three independent biological repli- cates).
    Figure Legend Snippet: Fig. 6. Gain of function of TSP-1 confers longevity extension in C. elegans and thermal resilience in human cells. (A) Schematic of the transgene that produces tsp-1 gain-of-function OE lines (OE line 1 at low level, OE line 2 at high level as measured by qRT-PCR). ***P < 0.001. (B) Life-span curves of wild type and two representative tsp-1 OE lines at 28°C starting at L4. A total of 50% median and 16% maximal survival extension by overexpression of tsp-1 in wild type, ***P < 0.001 (n > 40 animals per condition). (C) Life-span curves of wild-type and tsp-1 OE lines at constant 20°C. A total of 63% median and 24% maximal life-span extension by overexpression of tsp-1 in wild type, ***P < 0.001 (n > 40 animals per condition). (D) Serial confocal fluorescence images showing tetraspanin web-like structures formed in the human cell line HEK293 by CMV promoter-driven expression of C. elegans tsp-1::GFP (bottom) but not GFP alone (top). Scale bar, 10 μm. (E) Quantification of survival rates after heat shock in HEK293 cells showing enhanced thermal resilience conferred by ectopic gain-of-function of C. elegans TSP-1::GFP. ***P < 0.001 (three independent biological repli- cates).

    Techniques Used: Quantitative RT-PCR, Over Expression, Fluorescence, Expressing



    Similar Products

    native tsp  (Proteintech)
    95
    Proteintech native tsp
    Fig. 1. Thermal stress induces <t>TSP-1</t> expression and tetraspanin web-like structure formation. (A) Representative bright field and epifluorescence images showing expression of an integrated <t>transgene</t> <t>tsp-1p::tsp-1::GFP</t> under temperatures and durations indicated. Scale bar, 100 μm. (B) Quantification of TSP-1::GFP fluorescence inten- sities under conditions indicated. ***P < 0.001 (n > 40 animals per condition). (C) Schematic of the tsp-1 gene structure, predicted membrane topology (by DeepTMHMM), and structure (by AlphaFold2) of TSP-1. (D) SDS-PAGE and native-PAGE Western blots showing heat-induced increase in abundance and formation of dimers and multimers (asterisk) by TSP-1::GFP. (E) Representative confocal fluorescence images showing high-resolution Z-stack views of tetraspanin web structure formed by TSP-1::GFP, with en- larged view at right bottom. Scale bar, 10 μm. a.u., arbitrary units.
    Native Tsp, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/native tsp/product/Proteintech
    Average 95 stars, based on 1 article reviews
    native tsp - by Bioz Stars, 2026-03
    95/100 stars
    		  Buy from Supplier
    native human thrombospondin protein (tsp)  (Millipore)
    90
    Millipore native human thrombospondin protein (tsp)
    Fig. 1. Thermal stress induces <t>TSP-1</t> expression and tetraspanin web-like structure formation. (A) Representative bright field and epifluorescence images showing expression of an integrated <t>transgene</t> <t>tsp-1p::tsp-1::GFP</t> under temperatures and durations indicated. Scale bar, 100 μm. (B) Quantification of TSP-1::GFP fluorescence inten- sities under conditions indicated. ***P < 0.001 (n > 40 animals per condition). (C) Schematic of the tsp-1 gene structure, predicted membrane topology (by DeepTMHMM), and structure (by AlphaFold2) of TSP-1. (D) SDS-PAGE and native-PAGE Western blots showing heat-induced increase in abundance and formation of dimers and multimers (asterisk) by TSP-1::GFP. (E) Representative confocal fluorescence images showing high-resolution Z-stack views of tetraspanin web structure formed by TSP-1::GFP, with en- larged view at right bottom. Scale bar, 10 μm. a.u., arbitrary units.
    Native Human Thrombospondin Protein (Tsp), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/native human thrombospondin protein (tsp)/product/Millipore
    Average 90 stars, based on 1 article reviews
    native human thrombospondin protein (tsp) - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 1. Thermal stress induces TSP-1 expression and tetraspanin web-like structure formation. (A) Representative bright field and epifluorescence images showing expression of an integrated transgene tsp-1p::tsp-1::GFP under temperatures and durations indicated. Scale bar, 100 μm. (B) Quantification of TSP-1::GFP fluorescence inten- sities under conditions indicated. ***P < 0.001 (n > 40 animals per condition). (C) Schematic of the tsp-1 gene structure, predicted membrane topology (by DeepTMHMM), and structure (by AlphaFold2) of TSP-1. (D) SDS-PAGE and native-PAGE Western blots showing heat-induced increase in abundance and formation of dimers and multimers (asterisk) by TSP-1::GFP. (E) Representative confocal fluorescence images showing high-resolution Z-stack views of tetraspanin web structure formed by TSP-1::GFP, with en- larged view at right bottom. Scale bar, 10 μm. a.u., arbitrary units.

    Journal: Science advances

    Article Title: Early-life stress triggers long-lasting organismal resilience and longevity via tetraspanin.

    doi: 10.1126/sciadv.adj3880

    Figure Lengend Snippet: Fig. 1. Thermal stress induces TSP-1 expression and tetraspanin web-like structure formation. (A) Representative bright field and epifluorescence images showing expression of an integrated transgene tsp-1p::tsp-1::GFP under temperatures and durations indicated. Scale bar, 100 μm. (B) Quantification of TSP-1::GFP fluorescence inten- sities under conditions indicated. ***P < 0.001 (n > 40 animals per condition). (C) Schematic of the tsp-1 gene structure, predicted membrane topology (by DeepTMHMM), and structure (by AlphaFold2) of TSP-1. (D) SDS-PAGE and native-PAGE Western blots showing heat-induced increase in abundance and formation of dimers and multimers (asterisk) by TSP-1::GFP. (E) Representative confocal fluorescence images showing high-resolution Z-stack views of tetraspanin web structure formed by TSP-1::GFP, with en- larged view at right bottom. Scale bar, 10 μm. a.u., arbitrary units.

    Article Snippet: Native TSP- 1::GFP were detected using antibodies against GFP (1:1000, 66002- 1- Ig, Proteintech) at cold room overnight.

    Techniques: Expressing, Fluorescence, Membrane, SDS Page, Clear Native PAGE, Western Blot

    Fig. 2. Expression and roles of TSP-1 in membrane barrier integrity in adults and during aging. (A to D) Representative epifluorescence images showing TSP-1::GFP up-regulation from L4 (A) to young adults (B) and during aging (C) and (D). (E) Quantification of fluorescence intensities of TSP-1::GFP under conditions indicated. ***P < 0.001 (n > 30 animals per condition). (F) Schematic of the assay for acute membrane barrier functions. (G) Quantification of intensities of fluorescein accumulated by live animals under conditions indicated. ***P < 0.001 (n > 20 animals per condition). (H to J) Representative epifluorescence images showing fluorescein accumulation in animals of indicated stages, temperature and genotypes (wild-type and tsp-1 deletion mutant allele ok3594). Scale bars, 100 μm.

    Journal: Science advances

    Article Title: Early-life stress triggers long-lasting organismal resilience and longevity via tetraspanin.

    doi: 10.1126/sciadv.adj3880

    Figure Lengend Snippet: Fig. 2. Expression and roles of TSP-1 in membrane barrier integrity in adults and during aging. (A to D) Representative epifluorescence images showing TSP-1::GFP up-regulation from L4 (A) to young adults (B) and during aging (C) and (D). (E) Quantification of fluorescence intensities of TSP-1::GFP under conditions indicated. ***P < 0.001 (n > 30 animals per condition). (F) Schematic of the assay for acute membrane barrier functions. (G) Quantification of intensities of fluorescein accumulated by live animals under conditions indicated. ***P < 0.001 (n > 20 animals per condition). (H to J) Representative epifluorescence images showing fluorescein accumulation in animals of indicated stages, temperature and genotypes (wild-type and tsp-1 deletion mutant allele ok3594). Scale bars, 100 μm.

    Article Snippet: Native TSP- 1::GFP were detected using antibodies against GFP (1:1000, 66002- 1- Ig, Proteintech) at cold room overnight.

    Techniques: Expressing, Membrane, Fluorescence, Mutagenesis

    Fig. 3. ELTS-triggered TSP-1::GFP is long-lasting and requires CBP-1. (A) Representative epifluorescence images showing long-lasting up-regulation of TSP-1::GFP upon transient ELTS (T28 for 24 hours at L4). (B) Representative epifluorescence images showing up-regulation of TSP-1::GFP upon sustained thermal stress (T28 for 24, 48, or 72 hours starting at L4). (C) Quantification of fluorescence intensities of TSP-1::GFP under conditions indicated. ***P < 0.001 (n > 30 animals per condition). (D) Schematic of experimental flow using RNAi screen to identify genes required for ELTS induction of TSP-1::GFP. TFs, transcription factors. (E) Representative bright-field and epifluorescence images showing that RNAi against tsp-1 or cbp-1 blocks up-regulation of TSP-1::GFP by ELTS. (F) Quantitative RT-PCR measurements of tsp-1 expres- sion levels under conditions indicated, showing transient tsp-1 up-regulation by ELTS in a CBP-1–dependent manner. **P < 0.01 and ***P < 0.001 (three independent biological replicates). Scale bars, 100 μm. n.s., not significant.

    Journal: Science advances

    Article Title: Early-life stress triggers long-lasting organismal resilience and longevity via tetraspanin.

    doi: 10.1126/sciadv.adj3880

    Figure Lengend Snippet: Fig. 3. ELTS-triggered TSP-1::GFP is long-lasting and requires CBP-1. (A) Representative epifluorescence images showing long-lasting up-regulation of TSP-1::GFP upon transient ELTS (T28 for 24 hours at L4). (B) Representative epifluorescence images showing up-regulation of TSP-1::GFP upon sustained thermal stress (T28 for 24, 48, or 72 hours starting at L4). (C) Quantification of fluorescence intensities of TSP-1::GFP under conditions indicated. ***P < 0.001 (n > 30 animals per condition). (D) Schematic of experimental flow using RNAi screen to identify genes required for ELTS induction of TSP-1::GFP. TFs, transcription factors. (E) Representative bright-field and epifluorescence images showing that RNAi against tsp-1 or cbp-1 blocks up-regulation of TSP-1::GFP by ELTS. (F) Quantitative RT-PCR measurements of tsp-1 expres- sion levels under conditions indicated, showing transient tsp-1 up-regulation by ELTS in a CBP-1–dependent manner. **P < 0.01 and ***P < 0.001 (three independent biological replicates). Scale bars, 100 μm. n.s., not significant.

    Article Snippet: Native TSP- 1::GFP were detected using antibodies against GFP (1:1000, 66002- 1- Ig, Proteintech) at cold room overnight.

    Techniques: Fluorescence, Quantitative RT-PCR

    Fig. 4. Subcellular localization and stability of tetraspanin web formed by endogenous TSP-1::wrmScarlet. (A) Representative confocal images showing colocaliza- tion of the marker Akt-PH::GFP, which binds to intestinal apical plasma membrane inner leaflet PIP2/PIP3, with endogenous TSP-1 tagged with wrmScarlet by CRISPR. Scale bars, 10 μm. (B) Fluorescent intensity correlation analysis showing close proximity of Akt-PH::GFP and TSP-1::wrmScarlet. (C) Representative confocal images showing non-colocalization of the marker ERm::GFP, which labels intestinal ER membrane, with endogenous TSP-1 tagged with wrmScarlet by CRISPR. Scale bars, 10 μm. (D) Fluo- rescent intensity correlation analysis for ERm::GFP and TSP-1::wrmScarlet. (E) Representative confocal time-series images showing stability of tetraspanin webs formed by endogenous TSP-1::wrmScarlet, with enlarged views in (F). Scale bars, 10 μm.

    Journal: Science advances

    Article Title: Early-life stress triggers long-lasting organismal resilience and longevity via tetraspanin.

    doi: 10.1126/sciadv.adj3880

    Figure Lengend Snippet: Fig. 4. Subcellular localization and stability of tetraspanin web formed by endogenous TSP-1::wrmScarlet. (A) Representative confocal images showing colocaliza- tion of the marker Akt-PH::GFP, which binds to intestinal apical plasma membrane inner leaflet PIP2/PIP3, with endogenous TSP-1 tagged with wrmScarlet by CRISPR. Scale bars, 10 μm. (B) Fluorescent intensity correlation analysis showing close proximity of Akt-PH::GFP and TSP-1::wrmScarlet. (C) Representative confocal images showing non-colocalization of the marker ERm::GFP, which labels intestinal ER membrane, with endogenous TSP-1 tagged with wrmScarlet by CRISPR. Scale bars, 10 μm. (D) Fluo- rescent intensity correlation analysis for ERm::GFP and TSP-1::wrmScarlet. (E) Representative confocal time-series images showing stability of tetraspanin webs formed by endogenous TSP-1::wrmScarlet, with enlarged views in (F). Scale bars, 10 μm.

    Article Snippet: Native TSP- 1::GFP were detected using antibodies against GFP (1:1000, 66002- 1- Ig, Proteintech) at cold room overnight.

    Techniques: Marker, Clinical Proteomics, Membrane, CRISPR

    Fig. 5. ELTS-triggered thermal resilience and longevity requires TSP-1. (A) Life-span curves of wild-type and tsp-1 mutants at 28°C starting at L4. A total of 20% me- dian and 28% maximal survival decrease in tsp-1 mutants. ***P < 0.001 (n > 40 animals per condition). (B) Life-span curves of wild-type and tsp-1 mutants at constant 20°C or after transient 28°C for 24 hours at L4 (ELTS). A total of 36% median and 20% maximal life-span extension by ELTS in wild type. *** P < 0.001 (n > 35 animals per condition). (C) Life-span curves of wild-type and tsp-1 mutants at 28°C after transient 28°C for 24 hours at L4 (ELTS). A total of 33% median and 33% maximal survival de- crease in tsp-1 mutants. ***P < 0.001 (n > 35 animals per condition). (D) Life-span curves of wild-type animals with RNAi against cbp-1 or control at 28°C starting at L4. A total of 80% median and 50% maximal survival decrease with RNAi against cbp-1. ***P < 0.001 (n > 40 animals per condition). (E) Representative confocal fluorescence images showing ELTS-induced increase in numbers of nuclei with GFP::CBP-1 in the posterior intestine. (F) Quantification of numbers of nuclei with GFP::CBP-1 in the posterior intestine under conditions indicated. (G) Model of how ELTS induces lasting organismal thermal resilience through HSF-independent epigenetic regulation of TSP-1 and stable tetraspanin web structure formation.

    Journal: Science advances

    Article Title: Early-life stress triggers long-lasting organismal resilience and longevity via tetraspanin.

    doi: 10.1126/sciadv.adj3880

    Figure Lengend Snippet: Fig. 5. ELTS-triggered thermal resilience and longevity requires TSP-1. (A) Life-span curves of wild-type and tsp-1 mutants at 28°C starting at L4. A total of 20% me- dian and 28% maximal survival decrease in tsp-1 mutants. ***P < 0.001 (n > 40 animals per condition). (B) Life-span curves of wild-type and tsp-1 mutants at constant 20°C or after transient 28°C for 24 hours at L4 (ELTS). A total of 36% median and 20% maximal life-span extension by ELTS in wild type. *** P < 0.001 (n > 35 animals per condition). (C) Life-span curves of wild-type and tsp-1 mutants at 28°C after transient 28°C for 24 hours at L4 (ELTS). A total of 33% median and 33% maximal survival de- crease in tsp-1 mutants. ***P < 0.001 (n > 35 animals per condition). (D) Life-span curves of wild-type animals with RNAi against cbp-1 or control at 28°C starting at L4. A total of 80% median and 50% maximal survival decrease with RNAi against cbp-1. ***P < 0.001 (n > 40 animals per condition). (E) Representative confocal fluorescence images showing ELTS-induced increase in numbers of nuclei with GFP::CBP-1 in the posterior intestine. (F) Quantification of numbers of nuclei with GFP::CBP-1 in the posterior intestine under conditions indicated. (G) Model of how ELTS induces lasting organismal thermal resilience through HSF-independent epigenetic regulation of TSP-1 and stable tetraspanin web structure formation.

    Article Snippet: Native TSP- 1::GFP were detected using antibodies against GFP (1:1000, 66002- 1- Ig, Proteintech) at cold room overnight.

    Techniques: Control, Fluorescence

    Fig. 6. Gain of function of TSP-1 confers longevity extension in C. elegans and thermal resilience in human cells. (A) Schematic of the transgene that produces tsp-1 gain-of-function OE lines (OE line 1 at low level, OE line 2 at high level as measured by qRT-PCR). ***P < 0.001. (B) Life-span curves of wild type and two representative tsp-1 OE lines at 28°C starting at L4. A total of 50% median and 16% maximal survival extension by overexpression of tsp-1 in wild type, ***P < 0.001 (n > 40 animals per condition). (C) Life-span curves of wild-type and tsp-1 OE lines at constant 20°C. A total of 63% median and 24% maximal life-span extension by overexpression of tsp-1 in wild type, ***P < 0.001 (n > 40 animals per condition). (D) Serial confocal fluorescence images showing tetraspanin web-like structures formed in the human cell line HEK293 by CMV promoter-driven expression of C. elegans tsp-1::GFP (bottom) but not GFP alone (top). Scale bar, 10 μm. (E) Quantification of survival rates after heat shock in HEK293 cells showing enhanced thermal resilience conferred by ectopic gain-of-function of C. elegans TSP-1::GFP. ***P < 0.001 (three independent biological repli- cates).

    Journal: Science advances

    Article Title: Early-life stress triggers long-lasting organismal resilience and longevity via tetraspanin.

    doi: 10.1126/sciadv.adj3880

    Figure Lengend Snippet: Fig. 6. Gain of function of TSP-1 confers longevity extension in C. elegans and thermal resilience in human cells. (A) Schematic of the transgene that produces tsp-1 gain-of-function OE lines (OE line 1 at low level, OE line 2 at high level as measured by qRT-PCR). ***P < 0.001. (B) Life-span curves of wild type and two representative tsp-1 OE lines at 28°C starting at L4. A total of 50% median and 16% maximal survival extension by overexpression of tsp-1 in wild type, ***P < 0.001 (n > 40 animals per condition). (C) Life-span curves of wild-type and tsp-1 OE lines at constant 20°C. A total of 63% median and 24% maximal life-span extension by overexpression of tsp-1 in wild type, ***P < 0.001 (n > 40 animals per condition). (D) Serial confocal fluorescence images showing tetraspanin web-like structures formed in the human cell line HEK293 by CMV promoter-driven expression of C. elegans tsp-1::GFP (bottom) but not GFP alone (top). Scale bar, 10 μm. (E) Quantification of survival rates after heat shock in HEK293 cells showing enhanced thermal resilience conferred by ectopic gain-of-function of C. elegans TSP-1::GFP. ***P < 0.001 (three independent biological repli- cates).

    Article Snippet: Native TSP- 1::GFP were detected using antibodies against GFP (1:1000, 66002- 1- Ig, Proteintech) at cold room overnight.

    Techniques: Quantitative RT-PCR, Over Expression, Fluorescence, Expressing